Using gluten index instrument to test wheat gluten index genotype and environmental difference

Genotypic and Environmental Differences of Wheat Gluten Index
1. Genotypic and environmental differences and significant analysis of gluten index. As can be seen from Table 2, there are differences between gluten index genotypes. From the test results of the gluten index instrument, the gluten index of winter wheat cultivars with different varieties was highest with PH1521, which was 1.49 times that of PH988-2 with the lowest gluten index. Among them, there was no significant difference between Youmai 2 and Jimai 20; there were significant or extremely significant differences among Other breeds. It can also be seen from Table 2 that there is little variation among most species sites, indicating that the genotype performance is relatively stable under different environmental conditions. Among them, PH988-2 has the largest coefficient of variation, which is 4.47 times that of Yanyou 361. It shows that there are great differences in the gluten index of PH988-2 under different environmental conditions. It has a wide range of variation and great potential for selection. It is possible to improve its gluten through breeding methods.

The average value of gluten index in different wheat varieties and its significance test
From Table 3, we can see that Tengzhou has the largest gluten index, which is significantly different from the other five locations; the Tai'an point has the smallest gluten index, which is also significantly different from other points. Among them, there is no significant difference between Texas and Jining, Boxing and Jining, and there are significant or extremely significant differences among other points. From Table 3, it can also be seen that there is little difference in the coefficient of variation between environments, indicating that the gluten index is not sensitive to the environment and the stability of the varieties is relatively large. The coefficient of variation of the gluten index was larger in different varieties at the same location, but there was no significant difference among the planting areas, indicating that the influence of environmental factors was relatively small, and the genotype was the main factor affecting the gluten index.

The average value of wheat gluten index in different locations and its significance test
2. Comparison of interannual differences in wheat gluten index. Figure 1 shows that in 2002, most varieties (lines) were higher in gluten index in Tengzhou (91.08), Dezhou (87.56), and Jining (85.26) than other points, and the lowest was Zhangqiu (75.63). There was no significant difference in the gluten index between the PH1521 and Jimei 20 environments, and the genotypes were stable and were good varieties. The other breeds had a large difference in the environment. The average gluten index of the six varieties (lines) was highest with PH1521 and lowest with PH988-2. This shows that there are significant differences in the gluten index between different planting regions and different varieties (lines), and the genotype effect is more significant. Therefore, in order to improve the gluten index, not only good quality and high stability varieties (lines) such as PH1521 should be selected, but also specific cultivation areas that are conducive to the growth of gluten index, such as Tengzhou and Dezhou, should be selected to achieve high quality production.

Variety index of wheat varieties in different experimental conditions
Figure 2 shows the gluten index of different wheat varieties in 2003. It can be seen that there are significant changes compared with 2002. The gluten index of most breeds (lines) was higher in Boxing (82.61) and Zhangqiu (82.35) than in other points, and lowest in Tengzhou (75.54). The average gluten index of the six varieties (lines) was similar to that of 2002, with the highest in PH1521 and the lowest in PH988-2. And PH1521 genotype stability is a good variety. From the results of the 2002 and 2003 experiments, it can be seen that the gluten index of each breed varies a little and has a certain degree of stability. In different circumstances, the gluten index is quite different.

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