Study on Vacuum Freeze-drying of Corneal Endothelial Cells

Study on Vacuum Freeze-drying of Corneal Endothelial Cells
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Vacuum freeze-drying preserved cornea has not been reported at home and abroad. Sucrose is difficult to dry when dry. According to the mechanism of the protective agent, we chose dimethyl sulfoxide (DMSO) as the protective agent inside the membrane and albumin as the protective agent outside the membrane. The purpose of drying is to remove the moisture in the corneal cells. After vacuum freeze-drying, the cornea can be easily aseptically packaged for long-term storage.

The discharge of water during corneal drying is complicated. To ensure the activity of corneal cells in the drying process, controlling the temperature, pressure, and drying rate of the drying process are three important factors. After the cornea is freeze-dried, it needs rehydration before use. After the cornea is vacuum freeze-dried, the corneal tissue structure and cell morphology are preserved, but the water loss, various ions and many nutrients are lost, and the vacuum freeze-drying The rehydration of the cornea is definitely different from the rewarming of the cornea after cryogenic freezing. Experimental Study on Vacuum Freeze - drying of Cell Culture Medium and Preservation of Rabbit Corneal Endothelial Cells .

The main function of corneal endothelial cells is to regulate the moisture in the cornea and maintain the transparency of the cornea. The corneal epithelium and endothelium were intact, smooth, transparent and no shedding after vacuum rehydration and corneal rehydration, and the whole cornea was free from edema and transparency. Microscopically, the corneal endothelial cells are hexagonal with clear boundaries.

It is believed that the damage is mainly manifested by the cells being highly sensitive to mechanical or osmotic injury. Vacuum freeze-drying will cause greater damage to the endothelial cells of the cornea. Although the density and activity rate of the corneal endothelial cells after rehydration after vacuum freeze-drying is lower than that in the deep cryopreservation group, it is lower than normal rabbits, but it is still higher s level. Vacuum freeze-drying may cause greater damage to the endothelial cells of the cornea than cryogenic freezing. However, the results suggest that vacuum freeze-drying can maintain the activity of corneal endothelial cells. With the continuous improvement of the quality and performance of the vacuum freeze dryer, the gradual improvement of the structure, the corneal endothelial cell density and survival rate will increase, creating a long-term preservation of the cornea.

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Max film covering width:300mm     heating power: 6kw 
Max process thickness: 90mm       overall dimensions:4000*600*1800mm
Min process length: 600mm         weight:2500kgs
Feeding speed:0-35m/min           max diameter of rolling cover material:400mm
Feeding motor power:1.5kw         max width of cover film 600/850/1200mm

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NO items
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